Media of Medical Laboratory Science

DETEKSI C-REACTIVE PROTEIN (CRP) PADA PENDERITA DIABETES MELITUS TERKONTROL : “LITERATURE REVIEW”

Putri — 2020-10-31

Diabetes Melitus (DM) merupakan suatu kelompok penyakit metabolik dengan karakteristik hiperglikemia yang terjadi karena kelainan sekresi insulin, kelainan kerja insulin atau kedua-duanya. Ketidaksesuaian kerja insulin pada penyakit DM ini mengakibatkan glukosa dari pembuluh darah tidak mampu masuk ke jaringan. adapun tujuan dari penelitian ini ialah untuk mengatahui gambaran C-reactive protein pada penderita Diabetes Melitus terkontrol dengan menggunakan metode aglutinasi latekx. Penelitian ini menggunakan study literatur dengan menggunakan jurnal nasional dan internasional yang di ambil berdasarkan kriteriainklusi dan ekslusi. Hasil penelitian ini ialah pada jurnal pertama terjadi peningkatan kadar CRP pada pasien DM tipe 2 dari golongan tidak rutin terapi sebagaipetanda adanya inflamasi. Pada jurnal kedua terjadi peningkatan kadar CRP pada penderita diabetes melitus tipe 2 sebagai petanda adanya proses inflamasi. Pada jurnal ketiga kadar CRP pada pasien diabetes tipe 2 dengan terapi insulin lebih rendah secara signifikan dibandingkan dengan menggunakan OHO. Pada jurnal keempat CRP pada pasien diabetes melitus tipe 2 yang positif sebanyak 14 pasien (70%) dan hasil yang negative sebanyak 6%. Pada jurnal kelima ada penurunan yang signifikan dalam serum MDA dan peningkatan yang signifikan pada TAS serum, tanpa efek yang signifikan dalam kadar hs-CRP setelah terapi metforman.

Skrining Jenis Bakteri Pada Penderita Cairan Telinga Otitis Media Akut (OMA) Di Kecamatan Woja Kabupaten Dompu

Efi Julia — 2020-10-31

Acute otitis media (OMA) is still a special health problem in children. Acute otitis media is an acute inflammation of the middle ear that occurs, especially in children. The purpose of this study was to determine the type of bacteria present in the ear fluid in patients with OMA in Woja District, Dompu Regency. A diagnosis of AOM can be confirmed by a careful history and examination. This research is explorative descriptive research. This research method was carried out by identifying the types of bacteria found in the ear fluid of patients with AOM. By making macroscopic and microscopic observations and biochemical tests the number of samples in this study were five people. Based on the results of this study, it was found there were five types of bacteria namely Proteus mixofaciens bacteria with microscopic features of beige page, small round shape, flat edges, smooth surface, stem cell morphology. Pseudomonas Sp with white microscopic features, round shape, flat edges, convex surface, stem cell morphology. Mirabilis protein with white microscopic features, round shape, flat edges, smooth surface, morphology of stem cells. Proteus Sp with microscopic characteristics of turbid yellow color, round shape, flat edges, smooth surface, morphology of stem cells. Klebsiella sp with microscopic features creamy turbid color, round shape, flat edges, smooth surface, morphology of the stem cells to five types of bacteria are included into gram-negative and rod-shaped bacteria. Biochemical test results showed that on average all bacteria isolated from OMA samples fermented sugar and differed only in certain types of sugar. From the results of screening research on the type of bacteria in the ear fluid of patients with acute otitis media (OMA) that has been done, found five types of bacteria that cause infection, three from the Proteus group, one from the Pseudomonas group, and one from the Klebsiella group.

ANTIDIABETIC ACTIVITIES OF PEGAGAN LEAF EXTRACT Centella assiatica L. AGAINST HYPERGLICEMIA MENCIT INDUCED ALOKSAN

Faizah — 2022-10-31

Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia due to abnormalities in insulin secretion, insulin action, or both. Pegagan leaf (Centella assiatica L.) contain flavonoids, saponins and tannins. Hypoglycemic activity on these flavonoids, saponins and tannins has potential as an antidiabetic. This study aims to determine the effect of giving pegagan leaf extract (Centella asiatica L.) to hyperglycemic male mice (Mus musculus L.) induced by alloxan. This research was a true experiment with the post test only control group design for 5 treatment groups, namely K-, K +, P1 (300 mg / kgBB), P2 (350 mg / kgBB) and P3 (400 mg / kgBB). Each group consisted of 5 mencit, so the total number of mice was 25 mencit. The results of this study were the average level of GDP decreased on day 15 for K + (Glibenclamide dose of 0.65 mg / kgBW) of 102.2 mg / dl, P1 (extract of gotu kola leaf dose of 300 mg / kgBB) of 127.75. mg / dl, P2 (pegagan leaf extract dose 350 mg / kgBB) of 119.5 mg / dl and P3 (pegagan leaf extract dosage of 400 mg / kgBB) of 107.4 mg / dl. The results of analysis of variance (ANOVA) showed a p value (Sig.) Of 0.008 <0.05. This means that there is a difference in the average level of GDP between various treatments. The results of the post hoc test show that the K- is significantly different from K +, P1, P2 and P3, but K + is not significantly different from P1, P2 and P3. So it can be concluded that the effect of giving pegagan leaf extract is that it can reduce GDP levels in hyperglycemic mencit.

Effectiveness of Network Leaf Extract (Urtica dioica L) to Resist Growth Streptococcus mutans Bacteria

Baiq Nurul Hidayah — 2020-10-31

Streptococcus mutans is a bacterium that causes dental caries. Dental caries is the most common dental and oral health problem in Indonesia. An alternative way to treat Streptococcus mutans is by using nettle leaf (Urtica dioica L) which is used by the community ethnopharmacologically as a traditional painkiller/analgesic drug. Nettle leaves contain compounds that are antibacterial such as alkaloids and terpenoids. The purpose of this research is to find out nettle leaf extract (Urtica dioica L) can inhibit the growth of Streptococcus mutans bacteria. This study is an experimental study using a post test only control group design with the well diffusion method with 4 treatment groups. Each petri dish was filled with MHA and inoculated with Streptococcus mutans, then 6 wells were made using a sterile stainless-steel bore (5 mm diameter). Extracts are made in various concentrations. Then it was incubated at 37°C for 24 hours and then the inhibition zone was measured using a caliper. The results of the study using the diffusion test method of the well, each treatment with various concentrations showed no inhibition of the growth of Streptococcus mutans bacteria on MHA (Mueller Hiton Agar) media. From this study it can be concluded that nettle leaf extract (Urtica dioica L) has no antibacterial activity because it has not been able to inhibit the growth of Streptococcus mutans bacteria.

PARE (MOMORDICA CHARANTIA) FRUIT EXTRACT ANTI-BACTERIAL ACTIVITY TEST AGAINST PSEUDOMONAS AERUGINOSA BACTERIA

Nadia Faeruz — 2020-10-31

Pare fruit contains flavonoids which function as antibacterial by forming complex compounds against extracellular proteins that disrupt the integrity of bacterial cell membranes. This study aims to determine the effect of giving the ethanol extract of bitter melon fruit to the Pseudomonas aeruginosa bacteria. This type of research is experimental research. The research design used was the post only control group design. This study used a sample of bitter melon with a concentration of 5%, 4,2%, 2,5%, 1,7% for 6 treatments and 4 repetitions, while for positive control (Ciprofloxacin). And negative control (VCO). From the research that has been done, it is found that the bitter melon extract does not have an inhibitory power against the growth of the bacteria Pseudomonas aeruginosa. The absence of an inhibition zone in the bitter melon extract was caused by factors of differences in the inhibitory test method, the extract drying method used, the extract concentration, and the difference in the content of the bitter melon fruit.